Monday - 9:30 - 17:30

 

 

 

-Participant and instructor introductions, housekeeping and course overview

 

-Hands-on introduction to UNIX and command line computing

 

-The history and ecology of eDNA. Overview of applications across disciplines

 

-Hands-on introduction to R

 

 

 

 

Tuesday - 9:30 - 17:30

 

 

 

-Theoretical introduction to single species detection (qPCR, ddPCR etc.)

 

-Hands-on design of qPCR assays

 

 

 

 

Wednesday  -  9:30 - 17:30

 

 

 

-Introduction to metabarcoding

 

-Hands-on analysis of metabarcoding data using OTU clustering

 

-Hands-on analysis of metabarcoding data using DADA2 denoiser

 

 

 

 

Thursday -  9:30 - 17:30

 

 

 

-Quality control and dataset refinement of metabarcoding data

 

-Assembling and curating reference libraries

 

-Taxonomic assignment of unknown DNA sequences

 

-Using high-throughput sequencing to answer ecological questions

 

 

 

 

Friday -  9:30 - 17:30

 

 

-Project day